Errors in "author determined" bio assy?

EM
Eric Martz
Thu, Jun 13, 2013 8:39 PM

It is my impression that when the "author determined" biological
assembly (REMARK 350) specifies the same structure as the asymmetric
unit, sometimes the authors simply forgot to specify a known assembly
during PDB deposition. Does anyone know of PDB entries where this has occurred?

Thanks, Eric

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It is my impression that when the "author determined" biological assembly (REMARK 350) specifies the same structure as the asymmetric unit, sometimes the authors simply forgot to specify a known assembly during PDB deposition. Does anyone know of PDB entries where this has occurred? Thanks, Eric /* - - - - - - - - - - - - - - - - - - - - - - - - - - - Eric Martz, Professor Emeritus, Dept Microbiology U Mass, Amherst -- http://Martz.MolviZ.Org Top Five 3D MolVis Technologies http://Top5.MolviZ.Org FirstGlance: 3D Views in Nature Structure - http://firstglance.jmol.org 3D Wiki with Scene-Authoring Tools http://Proteopedia.Org Biochem 3D Education Resources http://MolviZ.org ConSurf - Find Conserved Patches in Proteins: http://consurf.tau.ac.il Atlas of Macromolecules: http://atlas.molviz.org Interactive Molecules in Public Spaces http://MolecularPlayground.Org Workshops: http://workshops.molviz.org - - - - - - - - - - - - - - - - - - - - - - - - - - - */
JD
Jose Duarte
Fri, Jun 14, 2013 1:15 PM

Hi Eric

Here are a few cases for which the REMARK 350 annotated by authors says
the protein is a monomer but the authors in the paper say that the
proteins are actually dimers and prove it with independent experimental
methods (like AUC, gel filtration or light scattering):

3pc4
1wxi
1w3u
2ex2
2fwv
1mdo
1ytq
1v2x

Thus my guess is that these cases are most likely authors annotating a
monomer by default, since the asymmetric unit is monomeric.

Hope it helps

Jose


Jose Duarte
Laboratory of Biomolecular Research
Paul Scherrer Institute
5232 Villigen PSI
Switzerland
http://www.psi.ch/lbr/capitani_-guido

On 06/13/2013 10:39 PM, Eric Martz wrote:

It is my impression that when the "author determined" biological
assembly (REMARK 350) specifies the same structure as the asymmetric
unit, sometimes the authors simply forgot to specify a known assembly
during PDB deposition. Does anyone know of PDB entries where this has occurred?

Thanks, Eric

/* - - - - - - - - - - - - - - - - - - - - - - - - - - -
Eric Martz, Professor Emeritus, Dept Microbiology
U Mass, Amherst -- http://Martz.MolviZ.Org

Top Five 3D MolVis Technologies http://Top5.MolviZ.Org
FirstGlance: 3D Views in Nature Structure - http://firstglance.jmol.org
3D Wiki with Scene-Authoring Tools http://Proteopedia.Org
Biochem 3D Education Resources http://MolviZ.org
ConSurf - Find Conserved Patches in Proteins: http://consurf.tau.ac.il
Atlas of Macromolecules: http://atlas.molviz.org
Interactive Molecules in Public Spaces http://MolecularPlayground.Org
Workshops: http://workshops.molviz.org

                                                      • */

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Hi Eric Here are a few cases for which the REMARK 350 annotated by authors says the protein is a monomer but the authors in the paper say that the proteins are actually dimers and prove it with independent experimental methods (like AUC, gel filtration or light scattering): 3pc4 1wxi 1w3u 2ex2 2fwv 1mdo 1ytq 1v2x Thus my guess is that these cases are most likely authors annotating a monomer by default, since the asymmetric unit is monomeric. Hope it helps Jose --- Jose Duarte Laboratory of Biomolecular Research Paul Scherrer Institute 5232 Villigen PSI Switzerland http://www.psi.ch/lbr/capitani_-guido On 06/13/2013 10:39 PM, Eric Martz wrote: > It is my impression that when the "author determined" biological > assembly (REMARK 350) specifies the same structure as the asymmetric > unit, sometimes the authors simply forgot to specify a known assembly > during PDB deposition. Does anyone know of PDB entries where this has occurred? > > Thanks, Eric > > > /* - - - - - - - - - - - - - - - - - - - - - - - - - - - > Eric Martz, Professor Emeritus, Dept Microbiology > U Mass, Amherst -- http://Martz.MolviZ.Org > > Top Five 3D MolVis Technologies http://Top5.MolviZ.Org > FirstGlance: 3D Views in Nature Structure - http://firstglance.jmol.org > 3D Wiki with Scene-Authoring Tools http://Proteopedia.Org > Biochem 3D Education Resources http://MolviZ.org > ConSurf - Find Conserved Patches in Proteins: http://consurf.tau.ac.il > Atlas of Macromolecules: http://atlas.molviz.org > Interactive Molecules in Public Spaces http://MolecularPlayground.Org > Workshops: http://workshops.molviz.org > - - - - - - - - - - - - - - - - - - - - - - - - - - - */ > > > TO UNSUBSCRIBE OR CHANGE YOUR SUBSCRIPTION OPTIONS, please see > https://lists.sdsc.edu/mailman/listinfo.cgi/pdb-l .